Research Laboratory of Excessive Calcification Diseases

Head of Laboratory: Dmitry Tolpygin, MD, PhD.

Major tasks

To organize and carry out preclinical and clinical studies in the field of congenital and acquired diseases of the aorta and aortic valve.
To provide Information support and coordinate the interaction of the Centre’s research teams with users of research products in order to actively promote the Centre’s developments in the market of intellectual services, high technologies and search for investors.

Focus areas

Molecular and genetic studies of the mechanisms underlying the development of congenital and acquired diseases of the aorta and aortic valve.
Systematizing, improving and developing new methods for diagnosing the diseases of the aorta and aortic valve.
Developing and introducing into clinical practice new technologies and approaches to surgical, transcatheter and hybrid treatment of diseases of the aorta and aortic valve.
Carrying out a long-term follow-up of patients after various surgical procedures on the aorta and aortic valve as well as those receiving medical treatment.

Project

Searching for new directions of targeted therapy for congenital and acquired diseases with excessive calcification.

Structure

1. Research Group of Molecular Mechanisms of Calcification

Anna Malashicheva, DSc in Biology, Head of Group
Arseny Lobov, PhD in Biology, Researcher
Nadezhda Boyarskaya, Junior Researcher
Anastasia Shishkova, Junior Researcher
Pavel Dokshin, Junior Researcher
Olga Kachanova, Research Assistant

2. Biological Modelling Research Group

Pavel Kvindt, Junior Researcher

Major publications

Kozyrev I., Dokshin P., Kostina A, et al. Dysregulation of Notch signaling in cardiac mesenchymal cells of patients with tetralogy of Fallot. Pediatr Res. 2020;(November 2019):1-10. doi:10.1038/s41390-020-0760-6 IF 2,7, Q1
Malashicheva A., A. Kostina, A. Kostareva, O. Irtyuga, M. Gordeev and V. Uspensky (2020). “Notch signaling in the pathogenesis of thoracic aortic aneurysms: A bridge between embryonic and adult states.” Biochim Biophys Acta Mol Basis Dis 1866(3): 165631. https://doi.org/10.1016/j.bbadis.2019.165631 IF 4,3 Q1
Aquila G., Kostina A., Vieceli Dalla Sega F., Shlyakhto E., Kostareva A., Marracino L., Ferrari R., Rizzo P., Malaschicheva A. 2019 The Notch pathway: a novel therapeutic target for cardiovascular diseases? Expert Opin Ther Targets 23:695–710. https://doi.org/10.1080/14728222.2019.1641198 IF 4,6, Q1
Semenova, D., M. Bogdanova, A. Kostina, A. Golovkin, A. Kostareva and A. Malashicheva (2020). “Dose-dependent mechanism of Notch action in promoting osteogenic differentiation of mesenchymal stem cells.” Cell Tissue Res 379(1): 169–179. DOI: 10.1007/s00441-019–03130 7 IF 3,4, Q1
Perepelina K., Klauzen P., Kostareva A., Malashicheva A. Tissue-Specific Influence of Lamin A Mutations on Notch Signaling and Osteogenic Phenotype of Primary Human Mesenchymal Cells. Cells. 2019 Mar 21;8(3). doi: 10.3390/cells8030266 IF 5,6 Q1
Kostina A., Shishkova A., Ignatieva E., Irtyuga O., Bogdanova M., Levchuk K., Golovkin A., Zhiduleva E., Uspenskiy V., Moiseeva O., Faggian G., Vaage J., Kostareva A., Rutkovskiy A., Malashicheva A. Different Notch signaling in cells from calcified bicuspid and tricuspid aortic valves. J Mol Cell Cardiol. 2018 114:211–219. doi: 10.1016/j.yjmcc.2017.11.009. IF 5,68 Q1
Ignatieva E., Kostina D., Irtyuga O., Uspensky V., Golovkin A., Gavriliuk N., Moiseeva O., Kostareva A., Malashicheva A.. Mechanisms of Smooth Muscle Cell Differentiation Are Distinctly Altered in Thoracic Aortic Aneurysms Associated with Bicuspid or Tricuspid Aortic Valves. Front Physiol. 2017 Jul 25;8:53 https://www.frontiersin.org/articles/10.3389/fphys.2017.00536/full IF 4,1 Q1
Rutkovskiy A., Malashicheva A., Sullivan G., Bogdanova M., Kostareva A., Stensløkken KO., Fiane A., Vaage J. Valve Interstitial Cells: The Key to Understanding the Pathophysiology of Heart Valve Calcification. J Am Heart Assoc. 2017 Sep 14;6(9). pii: e006339. doi: 10.1161/JAHA.117.006339. IF 5,1 Q1
Kostina D., Zverev D., Gordeev M., Ignatieva E., Voronkina I., Kostareva A., Malashicheva A. Aortic graft at coronary artery bypass surgery as a source of human aortic smooth muscle cells. Cell Transplant. 2017 Oct;26(10):1663–1668. doi: 10.1177/0963689717721226. IF 3,2 Q1
Kostina A. S., Uspensky V. Е., Irtyuga O. B., Ignatieva E. V., Freylikhman O., Gavriliuk N. D., Moiseeva O. M., Zhuk S., Tomilin A., Kostareva А. А., Malashicheva A. B. Notch-dependent EMT is attenuated in patients with aortic aneurysm and bicuspid aortic valve. Biochim Biophys Acta. 2016 Apr;1862(4):733–40 http://www-ncbi-nlm-nih-gov.proxy.kib.ki.se/pubmed/26876948 IF 5,2 Q1

Patents and inventions

Contacts

Dmitry Tolpygin, Head of Laboratory
e-mail: tolpygin_ds@almazovcentre.ru